Immunocytochemical Evidence for Glucagon-Containing Cells in the Human Stomach

L. Munöz Barragan; C. Rufener; C. B. Srikant; R. E. Dobbs; W. A. Shannon Jr.; D. Baetens; R. H. Unger

doi:10.1055/s-0028-1093580

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 1977; 9(1): 37-39
DOI: 10.1055/s-0028-1093580

Originals

Immunocytochemical Evidence for Glucagon-Containing Cells in the Human Stomach[*]

L. Munöz Barragan^** , C. Rufener , C. B. Srikant , R. E. Dobbs , W. A. Shannon Jr. , D. Baetens , R. H. Unger

The Dallas Veterans Administration Hospital; The University of Texas Southwestern Medical School at Dallas, Texas, U.S.A.; and the Institute of Histology and Embryology, University of Geneva Medical School, Geneva, Switzerland

Abstract

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Abstract

To determine if glucagon-containing cells could be identified in the human fundus, stomachs attained at autopsy within 4-hours of death from persons previously considered to be in good health were examined by the indirect immunoperoxidase technique using antiglucagon serum 30K.

Glucagon-containing cells were demonstrated in one of eight gastric fundi examined. The glucagon content of acid alcohol extracts of the fundi was low in all cases.

Glucagon content was also low in canine stomach removed 4-hours after death.

It is concluded that glucagon-containing cells, demonstrable by immunocytochemical techniques, may be present in the gastric fundus of humans.

Key words

Glucagon - A-Cells - Gastric Fundus - Immunocytochemistry - Immunoperoxidase

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References

1 This work was supported by N.I.H. Grant AM 02700-15, and Fonds National Suisse de la Recherche Scientifique " Grant 3.553.75.

2 Present address: Department of Anatomy, Faculty of Medicine, Salamanca, Spain.